English
norskBlar i forfatter "Coucheron, David Andre"
Viser treff 1-13 av 13
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Chip Based Nanoscopy: Towards Integration and High-throughput Imaging
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017)Super-resolution optical microscopy, commonly referred to as optical nanoscopy, has enabled imaging of biological samples with a resolution that was only achievable previously using electron microscopy. Optical nanoscopy is a rapidly growing field, with several different techniques and implementations that overcome the diffraction limit of light. However, the common nanoscope continues to be a rather ... -
Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-24)Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used in histological investigations, their resolving capabilities are insufficient to visualize details at subcellular level. Although a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases, the ... -
Chip-Based Resonance Raman Spectroscopy Using Tantalum Pentoxide Waveguides
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-08)Blood analysis is an important diagnostic tool, as it provides a wealth of information about the patient's health. Raman spectroscopy is a promising tool for blood analysis, but widespread clinical application is limited by its low signal strength, as well as complex and costly instrumentation. The growing field of waveguide-based Raman spectroscopy tries to solve these challenges by working toward ... -
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-11-16)Total internal reflection fluorescence (TIRF) is commonly used in single molecule localization based super-resolution microscopy as it gives enhanced contrast due to optical sectioning. The conventional approach is to use high numerical aperture microscope TIRF objectives for both excitation and collection, severely limiting the field of view and throughput. We present a novel approach to generating ... -
Kontinuitet i overgangen fra skole til universitet - Implikasjoner av Fagfornyelsen for MNT-fagene
(Conference object; Konferansebidrag, 2023-03)Med LK20 Fagfornyelsen innføres det nye læreplaner i skolen, der antall kompetansemål i de ulike fagene er noe redusert for å gi mer rom til dybdelæring, samt at læreplanene i matematikk og fysikk har et større fokus på utforsking, modellering og bruk av numeriske metoder enn tidligere (Ludvigsen et al., 2015; Utdanningsdirektoratet, 2020, 2021). I dette bidraget presenterer vi hvordan en gruppe ... -
Multimodal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cells
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-23)Visualization of three-dimensional (3D) morphological changes in the subcellular structures of a biological specimen is a major challenge in life science. Here, we present an integrated chip-based optical nanoscopy combined with quantitative phase microscopy (QPM) to obtain 3D morphology of liver sinusoidal endothelial cells (LSEC). LSEC have unique morphology with small nanopores (50-300 nm in ... -
Nanoscopy on-a-chip: super-resolution imaging on the millimeter scale
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-04)Optical nanoscopy techniques can image intracellular structures with high specificity at sub-diffraction limited resolution, bridging the resolution gap between optical microscopy and electron microscopy. So far conventional nanoscopy lacks the ability to generate high throughput data, as the imaged region is small. Photonic chip-based nanoscopy has demonstrated the potential for imaging large areas, ... -
Photonic-chip: a multimodal imaging tool for histopathology
(Conference object; Konferansebidrag, 2021-04)We propose the photonic-chip as a multimodal imaging platform for histopathological assessment, allowing large fields-of-view across diverse microscopy methods including total internal reflection fluorescence and single-molecule localization. -
Self-assessment of written exercises in STEM courses
(Conference object; Konferansebidrag, 2023-03)Students of physics in Norway typically spend a lot of time on mandatory problemsolving exercises that they must get a passing grade on to be allowed to take the exam. This is often a primarily summative assessment, which can limit the learning potential. <p> <p>In this contribution, we present an intervention in which traditional mandatory assignments are modified to put more emphasis on ... -
Sex-specific height-correction of weight in a population with ethnic groups that differ in stature - the SAMINOR 1 Survey: a cross-sectional study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-07)Background: Body mass index (BMI, weight/height2) is a common proxy for body fatness, but it is negatively correlated with height. In Norway, the ethnic Sami people have had higher BMI and lower height than their non-Sami peers. This article aimed to examine if previous findings of higher obesity measures in Sami compared to non-Sami persist when applying an adequately height-corrected weight ... -
Studentaktiv læring med store studentgrupper – fordeler og ulemper med gruppediskusjon i pollbasert undervisning
(Conference object; Konferansebidrag, 2023-03)To viktige prinsipper for å øke studentenes læring i undervisning er aktiv deltagelse og formativ vurdering. Innføring av disse prinsippene i undervisning med store studentgrupper kan imidlertid by på utfordringer. Løsningen kan være å bruke flervalgsoppgaver i undervisningen med påfølgende formativ vurdering av faglæreren, en undervisningsform som vi her kaller pollbasert undervisning. I dette ... -
Study of waveguide background at visible wavelengths for on-chip nanoscopy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-06-17)On-chip super-resolution optical microscopy is an emerging field relying on waveguide excitation with visible light. Here, we investigate two commonly used high-refractive index waveguide platforms, tantalum pentoxide (Ta<sub>2</sub>O<sub>5</sub>) and silicon nitride (Si<sub>3</sub>N<sub>4</sub>), with respect to their background with excitation in the range 488–640 nm. The background strength from ... -
Waveguide-based Excitation for High-throughput Imaging
(Doctoral thesis; Doktorgradsavhandling, 2021-03-24)Chip–based fluorescence imaging is an emerging field where the fluorophores in a sample are excited by the evanescent field generated by waveguides. The aim of this thesis is to explore how waveguide–based excitation can benefit super–resolution optical microscopy, as well as investigate other imaging and spectroscopic applications which can benefit from the same platform. For all imaging and ...
